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[ View/Ask ]- QIAGEN Protease: A recombinant serine endopeptidase derived from Bacillus. It is supplied as a lyophilized (freeze-dried) powder to maximize long-term stability at room temperature.
- QIAGEN Protease Solvent: A specialized aqueous solution formulated with 0.04% sodium azide as a preservative. It is specifically used to reconstitute the lyophilized protease powder into a ready-to-use liquid enzyme.
- QIAamp DNA Blood (Manual) Kits: Reconstitute one vial of protease with 7.0 mL of solvent or distilled water.
- QIAamp BioRobot Kits: Reconstitute one vial of protease with 10.0 mL of distilled water. [1]
- Lyophilized Powder: Stable for up to 12 months when stored dry at room temperature (15–25°C). For longer shelf life, store at 2–8°C.
- Reconstituted Liquid: Stable for up to 2 months when stored at 2–8°C.
- Long-term Storage: Can be stored at –20°C for up to 6 months. It is highly recommended to divide the solution into aliquots before freezing to prevent activity loss from repeated freeze-thaw cycles.
- Optimal Conditions: The enzyme exhibits broad substrate specificity and remains highly active in alkaline conditions (pH 7.5–10.5) and elevated temperatures up to 55°C.
- Denaturant Compatibility: Its activity actually increases in the presence of common lysis buffers containing denaturants like urea, guanidine HCl, or up to 1% SDS.
- Inhibitor Resistance: It is not inhibited by up to 100 mM EDTA in standard buffers, allowing it to function while nucleases are safely deactivated.
- Inactivation: Easily inactivated by heating the reaction mixture to 70°C for 15 minutes.
- Target Residues: Protease cleaves preferentially at neutral and acidic amino acid residues, whereas Proteinase K targets the carboxyl side of hydrophobic, aliphatic, and aromatic residues.
- Digest Speed: Proteinase K is preferred for highly complex tissues requiring rapid digestion times.
- Cost: QIAGEN Protease serves as a highly economical alternative to Proteinase K for isolation from straightforward samples like liquid blood and cultured cells.
LIMITED PICKUP DATES/TIMES:
Currently, pickup dates and times are EXTREMELY LIMITED. Pickups will only be available to be scheduled on Thursdays of each week, and within a timeframe of 10:00am and 1:00pm. Scheduling a specific date/time is STILL REQUIRED! This notice supersedes all other pickup times given elsewhere in the auction information.
Please Note: All electronic items are untested. All sales are final, and no refund or exchange requests will be accepted. The photos below are example images, and the item you win may be slightly different than what is shown. The Pickup Location may be different than the one shown in the auction. The winner will be given the correct address once payment has been given.
**Notice to Bidders**
WE DO NOT SHIP ITEMS.
The winning bidder is responsible for supplying proper manpower, equipment (i.e. dolly, handtruck, etc.), and appropriate vehicles for pickup. No equipment or manpower will be supplied by the County of Sonoma. You are required to pick-up all items included in the auction.
Winning bidder must make an appointment, and confirm it(!), with the auction contact to arrange pick-up date/time. DO NOT come to the pickup location without an appointment! You will not receive your items if you do!
Please note that pickup time/date availability will vary, however, pickups are usually able to be scheduled on Thursdays, at a scheduled time between 10am and 1pm. Due to specific item locations and Auction Contact availability, exceptions may be made. If in doubt, please contact the Auction Contact for more information.
We do not ship items, so we do not accept any pre-paid shipping labels. If you are unable to pick up your auction winnings in person, you may specify a family member or friend to pick up the item for you, or, you may hire a courier/freight company to pick up, pack, and ship the item to you. If you require a courier/freight company to pick up your auction winnings, please note that 1) you must reach out to the Auction Contact and inform them of this before doing any pickup scheduling, and 2) we are unable to put items in boxes, or secure them to pallets, so the courier/freight company you chose must be able to do that themselves if needed.
Payment must be received within 5 calendar days of auction ending, or else the item(s) may be relisted. If the item(s) are not picked up within 3 weeks of the auction ending, the item(s) may be relisted, and no refunds will be given.
It is the responsibility of the Bidder to fully understand what it is you are bidding on. Some items can be extremely heavy, require a specific type of vehicle to transport, or take considerable time to remove. The County shall deny access to pick up auction winnings if the bidder arrives unprepared to SAFELY and expediently remove said property from the premises within the time allocated for the pickup, regardless of distance traveled or under any other circumstance.
If you cannot safely remove the items within the time allocated you will be asked to leave the premises. NO EXCEPTIONS WILL BE GRANTED, and NO REFUND provided. Do not arrive by yourself to pick up large or heavy items that require assistance. If you cannot safely load items purchased in the allocated time, you will be asked to leave the premises and either forfeit the transaction or arrange to come back within two business days for pickup if you still have pickup time available.
This item is sold as is, where is.
Viewing of Auction items by appointment only.
Public Processing, LLC handles all payments for County of Sonoma. Acceptable forms of payment are credit card. NO CASH, CHECKS, OR MONEY ORDERS WILL BE ACCEPTED!
Payment for an awarded item must be received within 5 calendar days of close of auction.
Sales Tax: County of Sonoma may charge 10.00% sales tax. The tax rate will be calculated at the time of bidding on an item(s). When sales tax is included, the buyer shall add and include the sales tax amount when making payment.
Buyers Premium: A 10% Buyers Premium will be added to the final sale price with a $1 minimum charge per auction to collect payment. This premium if applicable will be visible during the bidding process and added to the total bid.
All sales are final, and no refund or exchange requests will be accepted. All electronic items are untested. Public Surplus will notify buyer of receipt of payment via email. Buyer must remove auction item(s) from said agencies premises within 10 business days of notification by Public Surplus of the auction end. The payment receipt must be presented at the time of pick up or the item will not be released to you.
County of Sonoma may require bid deposits to ensure fairness and equity to all buyers as a way to decrease default bidding.
Pick-up hours by appt. only. Successful bidder will be responsible for removal of item. Shipping is not available, and the successful bidder must pick up their items in person or via courier.
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Public Processing, LLC handles all payments for County of Sonoma. Acceptable forms of payment are credit card. NO CASH, CHECKS, OR MONEY ORDERS WILL BE ACCEPTED!
Payment for an awarded item must be received within 5 calendar days of close of auction.
Sales Tax: County of Sonoma may charge 10% sales tax. The tax rate will be calculated at the time of bidding on an item(s). When sales tax is included, the buyer shall add and include the sales tax amount when making payment.
Buyers Premium: A 10% Buyers Premium will be added to the final sale price with a $1 minimum charge per auction to collect payment. This premium if applicable will be visible during the bidding process and added to the total bid.
All sales are final, and no refund or exchange requests will be accepted. All electronic items are untested. Public Surplus will notify buyer of receipt of payment via email. Buyer must remove auction item(s) from said agencies premises within 10 business days of notification by Public Surplus of the auction end. The payment receipt must be presented at the time of pick up or the item will not be released to you.
County of Sonoma may require bid deposits to ensure fairness and equity to all buyers as a way to decrease default bidding.
Pick-up hours by appt. only. Successful bidder will be responsible for removal of item. Shipping is not available, and the successful bidder must pick up their items in person or via courier